Radiographic dating definition
Seventeen days (median) after enrolment the rangers were randomized to DP or placebo 2 days before returning to forest camps where they stayed between 2 and 20 days (median 9.5 days).
One ranger in the DP-prophylaxis arm and one in the placebo arm were found to be infected with Plasmodium falciparum 15 days (median) after returning from the forest. falciparum isolates had molecular markers indicating resistance to artemisinins (K13-C580Y) and piperaquine (plasmepsin), but none had multiple copies of pfmdr1 associated with mefloquine resistance. | Show Abstract | Read more Malaria is one of the most important parasitic diseases of man.
Clinical episodes were recorded by village health workers.
The geographic coordinates (latitude and longitude) were collected for all houses and all participants were linked to their respective houses using a demographic surveillance system.
CONCLUSION: Anti-malarial prophylaxis in forest rangers is feasible. The development of drug resistance in malaria parasites is an inevitable consequence of their widespread and often unregulated use.
The findings of the study highlight the threat of multidrug-resistant malaria. There is an urgent need for new and effective drugs.
The developed method is suitable for implementation in high-throughput routine drug analysis, and was used to quantify pyronaridine accurately for up to 42days after a single oral dose in a drug-drug interaction study in healthy volunteers. Between August 2009 and February 2014, we conducted an open-label, randomized controlled trial of chloroquine (CQ) alone versus chloroquine plus primaquine (0.25 mg base/kg/day for 14 days) (CQ PQ) in patients aged 6 months and older with microscopy confirmed P. In the CQ PQ group, G6PD deficiency was excluded by fluorescent spot testing. vivax recurrence assessed by survival analysis over one year follow-up.
Results: Most clinical episodes and subclinical infections occurred within a single study village. Interventions that are dependent on predicting spatial clusters (such as reactive case detection) would only have detected a small proportion of cases unless the entire village was screened within a limited time frame and with a highly sensitive diagnostic test. | Show Abstract | Read more BACKGROUND: Prophylaxis for high-risk populations, such as forest workers, could be one component for malaria elimination in the Greater Mekong Sub-region.
Pyronaridine was extracted on a weak cation exchange solid-phase column (SPE) and separated on a HALO RP amide fused-core column using a gradient mobile phase of acetonitrile-ammonium formate and acetonitrile-methanol.
Detection was performed using electrospray ionization and tandem mass spectrometry (positive ion mode with selected reaction monitoring). | Show Abstract | Read more Afghanistan's national guidelines recommend primaquine (PQ) for radical treatment of Plasmodium vivax malaria, but this is rarely implemented because of concerns over potential hemolysis in patients who have G6PD deficiency.
We have developed a sensitive quantification method that covers a wide range of clinically relevant concentrations (1.5ng/m L to 882ng/m L) using a relatively low volume sample of 100μL of whole blood.
Total run time is 5min and precision is within ±15% at all concentration levels.
CONCLUSIONS: This study provides evidence of artemisinin resistance in a remote part of eastern Myanmar. | Show Abstract | Read more Background: The malaria burden is decreasing throughout the Greater Mekong Subregion, however transmission persists in some areas.